Current Member Publications

Postdoctoral Fellows and Associates

 

 

Logan Myler's Publications

L.R. Myler & I.J. Finkelstein (2017) Eukaryotic resectosomes: A single-molecule perspective. Prog Biophys Mol Biol 127: 119-129.

L.R. Myler, I.F. Gallardo, M.M. Soniat, R.A. Deshpande, X.B. Gonzalez, Y. Kim, T.T. Paull & I.J. Finkelstein (2017) Single-Molecule Imaging Reveals How Mre11-Rad50-Nbs1 Initiates DNA Break Repair. Mol Cell 67: 891-898.e4.

M.M. Soniat, L.R. Myler, J.M. Schaub, Y. Kim, I.F. Gallardo & I.J. Finkelstein (2017) Next-Generation DNA Curtains for Single-Molecule Studies of Homologous Recombination. Methods Enzymol 592: 259-281.

L.R. Myler, I.F. Gallardo, Y. Zhou, F. Gong, S.H. Yang, M.S. Wold, K.M. Miller, T.T. Paull & I.J. Finkelstein (2016) Single-molecule imaging reveals the mechanism of Exo1 regulation by single-stranded DNA binding proteins. Proc Natl Acad Sci U S A 113: E1170-9.

J.H. Lee, Z. Guo, L.R. Myler, S. Zheng & T.T. Paull (2014) Direct activation of ATM by resveratrol under oxidizing conditions. PLoS One 9: e97969.

 

  

Nanda Sasi Publications:

N.K. Sasi, A. Bhutkar, N.J. Lanning, J.P. Mackeigan & M. Weinreich (2017) DDK Promotes Tumor Chemoresistance and Survival via Multiple Pathways. Neoplasia 19: 439-450.

N.K. Sasi, K. Tiwari, F.F. Soon, D. Bonte, T. Wang, K. Melcher, H.E. Xu & M. Weinreich (2014) The potent Cdc7-Dbf4 (DDK) kinase inhibitor XL413 has limited activity in many cancer cell lines and discovery of potential new DDK inhibitor scaffolds. PLoS One 9: e113300.

L. Woodbine, J.A. Neal, N.K. Sasi, M. Shimada, K. Deem, H. Coleman, W.B. Dobyns, T. Ogi, K. Meek, E.G. Davies & P.A. Jeggo (2013) PRKDC mutations in a SCID patient with profound neurological abnormalities. J Clin Invest 123: 2969-2980.

 

Hiro Takai Publications:

Z. Mirman, F. Lottersberger, H. Takai, T. Kibe, Y. Gong, K. Takai, A. Bianchi, M. Zimmermann, D. Durocher & Titia de Lange (2018) 53BP1–RIF1–shieldin counteracts DSB resection through CST- and Polα-dependent fill-in.  Nature: 560: 112-116.

H. Takai, E. Jenkinson, S. Kabir, R. Babul-Hirji, N. Najm-Tehrani, D.A. Chitayat, Y.J. Crow & T. de Lange (2016) A POT1 mutation implicates defective telomere end fill-in and telomere truncations in Coats plusGenes Dev 30: 812-826.

E.A. Ahmed, A. Sfeir, H. Takai, and H. Scherthan (2013). Ku70 and non-homologous end joining protect testicular cells from DNA damage. J Cell Sci 126: 3095-3104.

P. Wu, H. Takai and T. de Lange (2012) Telomeric 3' Overhangs Derive from Resection by Exo1 and Apollo and Fill-In by POT1b-Associated CST. Cell 150: 39-52.

Z. Horejsi, H. Takai, S. Collins, H. Flynn, S. Maslen, J.M. Skehel, T. de Lange, and S. Boulton (2010) CK2 phospho-dependent binding of R2TP complex to Tel2 is essential for mTOR and SMG1 stability. Mol. Cell, 39:839-850.

H. Takai, Y. Xiey*, T. de Lange** and N.P. Pavletich** (2010) Tel2 is a helical repeat protein that mediates Hsp90-dependent maturation of mTOR and ATR complexes. Genes Dev. 24: 2019-2030.

H. Takai, R. Wang, K. Takai, H. Yang, and T. de Lange (2007) Tel2 regulates the stability of PI3K-related protein kinases. Cell 131: 1248-1259.

D. Hockemeyer, J.-P. Daniels, H. Takai, and T. de Lange (2006) Recent expansion of the telomeric complex in rodents: two distinct POT1 proteins protect mouse telomeres. Cell 126: 63-77.

L Cao, S Kim, C Xiao, R Wang, X Coumoul, X Wang, WM Li, X XuL, JA De Soto, H Takai, S Mai, SJ Elledge, N Motoyama, CX Deng. (2006) ATM-Chk2-p53 activation prevents tumorigenesis at an expense of organ homeostasis upon Brca1 deficiency. EMBO J 25: 2167-77.

J. Silverman, H. Takai, S.B.C. Buonomo, F. Eisenhaber, and T. de Lange (2004) Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint. Genes and Development 18: 2108-2119.

MT Jack, RA Woo, N Motoyama, H Takai, Lee PW. (2004) DNA-dependent protein kinase and checkpoint kinase 2 synergistically activate a latent population of p53 upon DNA damage. J Biol Chem 279: 15269-15273.

H. Takai, A. Smogorzewska, and T. de Lange (2003) DNA damage foci at dysfunctional telomeres. Curr. Biol. 13: 1549-1556.

J. Karlseder, L. Kachatrian, H. Takai, K. Mercer, S. Hingorani, T. Jacks, and T. de Lange (2003) Targeted deletion reveals an essential function for the telomere length regulator Trf1. Mol. Cell. Biol., 23: 6533-6541.

H Takai, K Naka, M Watanabe, Y Okada, H Sawa, M Nakanishi, H Suzuki, K Nagashima, K Ikeda, N Motoyama. (2002) Chk2-deficient mice exhibit radioresistance and defective p53-mediated transcription.  EMBO J  21: 5195-5205.

K Kobayashi, M Watanabe, Y Okada, H Takai, H Sawa, M Nakanishi, H Suzuki, K Nagashima, K Ikeda, N Motoyama. (2002) Hydrocephalus and male infertility in DNA polymerase alpha-deficient mice. Mol Cell Biol 22: 2769-2776.

T Sasaki, S Kamimura, H Takai, A Watanabe, K Onitake (2002) The activity for the induction of the sperm acrosome reaction localizes in the outer layers and exists in the high-molecular-weight components of the egg-jelly of the newt, Cynops pyrrhogaster. Zygote 10: 1-9.

H Takai, K Tominaga, N Motoyama, YA Minamishima, H Nagahama, T Tsukiyama, K Ikeda, K Nakayama, M Nakanishi, K Nakayma K. (2000) Aberrant cell cycle checkpoint function and early embryonic death in Chk1-/- mice. Genes Dev 14: 1439-1447.

M Kanematsu, T Sato, H Takai, K Watanabe, K Ikeda, Y Yamada. (2000) PGE2 induces expression of RANKL on pre-B cells: implications for accelerated osteoclastogenesis in estrogen deficiency.  J Bone Miner Res 15: 1321-1329.

K Onitake, H Takai, M Ukita, JI Mizuno, T Sasaki, A Watanabe. (2000) Significance of egg-jelly substances in the internal fertilization of the newt, Cynops pyrrhogaster. Comp Biochem Physiol B Biochem Mol Biol 126: 121-128.

H Mohri, K Inaba, M Kubo-Irie, H Takai, Y Yano-Toyoshima. (1999) Characterization of outer arm dynein in sea anemone, Anthopleira midori. Cell Motil Cytoskeleton 44: 202-208.

H Takai, M Kanematsu, K Yano, E Tsuda, K Higashio, K Ikeda, K Watanabe, Y Yamada. (1998) TGF-β stimulates the production of Osteoprotegerin/Osteoclastogenesis Inhibitory Factor by bone marrow stromal cells.  J Biol Chem 273: 27091-27096.

M Kanematsu, H Takai, Y Yamada. (1998) Actions of nitric oxide in the regulation of osteoclasts and osteoblasts. Curr Opinion Orthopedics 9: 11-16.

Y Yamada, A Miyauti, J Goto, Y Takagi, H Okuizumi, M Kanematsu, M Hase, H Takai, A Harada, K Ikeda. (1998) Association of a polymorphism of the Transforming Growth Factor β-1 gene with genetic susceptibility to osteoporosis in postmenopausal Japanese women. J Bone Miner Res 13: 1569-1576.

T Kameda, H Mano, Y Yamada, H Takai, N Amizuka, M Kobori, N Izumi, H Kawasima, H Ozawa, K Ikeda, A Kameda, Y Hakeda, M Kumegawa (1998) Calcium-sensing receptor in mature osteoclasts, which are bone resorbing cells.  Biochem Biophys Res Commun 245: 419-422.

K Ogawa, H Takai, A Ogiwara, E Yokota, T ShimizuT, K Inaba, H Mohri. (1996) Is outer arm dynein intermediate chain 1 multifunctional? Mol Biol Cell 7: 1895-1907.

H Shimizu, T Majima, H Takai, K Inaba, T Tomie (1995) Morphological changes of wrasse sperm axoneme after their motility initiation observed with use of atomic force microscopy. SPIE 2384: 45-51.

H Takai and M Morisawa (1995) Change in intracellular K+ concentration caused by external osmolality change regulates sperm motility of marine and freshwater teleosts.  J Cell Sci 108: 1175-1181.

 

John Zinder's Publications

E.M. Weick, M.R. Puno, K. Januszyk, J.C. Zinder, M.A. Dimattia & C.D. Lima (2018) Helicase-Dependent RNA Decay Illuminated by a Cryo-EM Structure of a Human Nuclear RNA Exosome-MTR4 Complex. Cell 173: 1663-1677.e21.

E.V. Wasmuth, J.C. Zinder, D. Zattas, M. Das & C.D. Lima (2017) Structure and reconstitution of yeast Mpp6-nuclear exosome complexes reveals that Mpp6 stimulates RNA decay and recruits the Mtr4 helicase. Elife 6.

J.C. Zinder & C.D. Lima (2017) Targeting RNA for processing or destruction by the eukaryotic RNA exosome and its cofactors. Genes Dev 31: 88-100.

J.C. Zinder, E.V. Wasmuth & C.D. Lima (2016) Nuclear RNA Exosome at 3.1 Å Reveals Substrate Specificities, RNA Paths, and Allosteric Inhibition of Rrp44/Dis3. Mol Cell 64: 734-745.

M.R. Wasserman, A. Pulk, Z. Zhou, R.B. Altman, J.C. Zinder, K.D. Green, S. Garneau-Tsodikova, J.H. Cate & S.C. Blanchard (2015) Chemically related 4,5-linked aminoglycoside antibiotics drive subunit rotation in opposite directions. Nat Commun 6: 7896.

E.M. Seco, J.C. Zinder, C.M. Manhart, A. Lo Piano, C.S. Mchenry & S. Ayora (2013) Bacteriophage SPP1 DNA replication strategies promote viral and disable host replication in vitro. Nucleic Acids Res 41: 1711-1721.

 

 

 

  

Graduate Students

 

Sarah Cai's Publications:

D.H. Lin, A.R. Correia, S.W. Cai, F.M. Huber, C.A. Jette & A.Hoelz (2018) Structural and functional analysis of mRNA export regulation by the nuclear pore complex. Nat Commun 9: 2319.

 

Alexander Stuart Publications: 

Coming soon!

 

Charles Kinzig Publications:

J.A. Brown, C.G. Kinzig, S.J. Degregorio & J.A. Steitz (2016) Hoogsteen-position pyrimidines promote the stability and function of the MALAT1 RNA triple helix. RNA 22: 743-749.

J.A. Brown, C.G. Kinzig, S.J. Degregorio & J.A. Steitz (2016) Methyltransferase-like protein 16 binds the 3’-terminal triple helix of MALAT1 long noncoding RNA. Proc Natl Acad Sci U S A 113: 14013-14018.

 

Zachary Mirman Publications:

Z. Mirman & T. de Lange (2020) 53BP1: a DSB escort.  Genes Dev. 34: 7-23.

Z. Mirman, F. Lottersberger, H. Takai, T. Kibe, Y. Gong, K. Takai, A. Bianchi, M. Zimmermann, D. Durocher & Titia de Lange (2018) 53BP1–RIF1–shieldin counteracts DSB resection through CST- and Polα-dependent fill-in.  Nature: 560: 112-116.

A.C. Paré, A. Vichas, C.T. Fincher, Z. Mirman, D.L. Farrell, A. Mainieri & J.A. Zallen (2014) A positional Toll receptor code directs convergent extension in Drosophila. Nature 515: 523-527.

 

 

Zhe Yang's Publications:

Z. Yang, K.K. Takai, C.A. Lovejoy & T. de Lange (2020) Break-induced replication promotes fragile telomere formation. Genes Dev 34: 1392-1405.

Z. Yang, J. Maciejowski & T. de Lange (2017) Nuclear envelope rupture is enhanced by loss of p53 or RbMol. Cancer Res. 15: 1579-1586.

K.K. Lim, T.Y. Ong, Y.R. Tan, E.G. Yang, B. Ren, K.S. Seah, Z. Yang, T.S. Tan, B.W. Dymock & E.S. Chen (2015) Mutation of histone H3 serine 86 disrupts GATA factor Ams2 expression and precise chromosome segregation in fission yeast. Sci Rep 5: 14064.

 

 

Audrey Goldbarb's Publications:

X. Tian, D. Firsanov, Z. Zhang, Y. Cheng, L. Luo, G. Tomblineet al. (2019) SIRT6 Is Responsible for More Efficient DNA Double-Strand Break Repair in Long-Lived Species. Cell 177: 622-638.e22.

 

 

George Zakusilo's Publications:

E. Devany, J.Y. Park, M.R. Murphy, G. Zakusilo, J. Baquero, X. Zhang, M. Hoque, B. Tian and F.E. Kleiman (2016) Intronic cleavage and polyadenylation regulates gene expression during DNA damage response through U1 snRNA. Cell Discov 2:16013.

 

 

  

Research Assistants

Kaori Takai Publications:

S.M. Dewhurst, X. Yao, J. Rosiene, H. Tian, J. Behr, N. Bosco, K.K. Takai, T. de Lange and M. ImieliƄski (2021) Structural variant evolution after telomere crisis. Nat Commun 12:2093.

Z. Yang, K.K. Takai, C.A. Lovejoy & T. de Lange (2020) Break-induced replication promotes fragile telomere formation. Genes Dev 34: 1392-1405.

I. Schmutz, A.R. Mensenkamp, K.K. Takai, M. Haadsma, L. Spruijt, R.M. de Voer, S.S. Choo, F.K. Lorbeer, E.J. van Grinsven, D. Hockemeyer, M.C. Jongmans and T. de Lange (2020) TINF2 is a haploinsufficient tumor suppressor that limits telomere length. Elife 9:e61235

C.A. Lovejoy, K. Takai, M.S. Huh, D.J. Picketts & T. de Lange (2020) ATRX affects the repair of telomeric DSBs by promoting cohesion and a DAXX-dependent activity. PLoS Biol 18: e3000594.

Z. Mirman, F. Lottersberger, H. Takai, T. Kibe, Y. Gong, K. Takai, A. Bianchi, M. Zimmermann, D. Durocher & Titia de Lange (2018) 53BP1–RIF1–shieldin counteracts DSB resection through CST- and Polα-dependent fill-in.  Nature: 560: 112-116.

K. Takai, T. Kibe, J. Donigian, D. Frescas and T. de Lange (2011) Telomere Protection by TPP1/POT1 Requires Tethering to TIN2. Mol. Cell, 44: 647-659.

K. Takai, S. Hooper, S. Blackwood, T. de Lange (2010) In Vivo stoichiometry of shelterin complex. J. Biol. Chem. 285: 1457-1467.

H. Takai, R. Wang, K. Takai, H. Yang, and T. de Lange (2007) Tel2 regulates the stability of PI3K-related protein kinases. Cell 131: 1248-1259.

D. Hockemeyer, W. Palm, T. Else, J.-P. Daniels, K. Takai, J. Z.-S. Ye, T. de Lange, and G. Hammer (2007) Telomere protection by mammalian POT1 requires interaction with TPP1. Nature Structural and Molecular Biology 8:754-61.

E Itakura, K Takai, K Umeda, M Kimura, M Ohsumi, K Tamai, and A Matsuura, (2004) Amino-terminal domain of ATRIP contributes to intranuclear relocation of the ATR-ATRIP complex following DNA damage. FEBS Lett 577: 289-293

C Kitazawa, K Takai, Y Nakajima, H Fujisawa, S Amemiya, (2004) LiCl inhibits the establishment of left-right asymmetry in larvae of the direct-developing echinoid Peronella japonica. J Exp Zool A Comp Exp Biol FEBS Lett Cell Struct Funct Hepatology 301: 707-17

K Takai, S Hattori, and S Irie, (2001) Type V collagen distribution in liver is reconstructed in coculture system of hepatocytes and stellate cells; the possible functions of type V collagen in liver under normal and pathological conditions. Cell Struct Funct 26: 289-302

C Tateno, K Takai-Kajihara, C Yamasaki, H Sato, and K Yoshizato, (2000) Heterogeneity of growth potential of adult rat hepatocytes in vitro. Hepatology 31: 65-74

 

Postdoctoral Fellows and Associates  Logan Myler's PublicationsL.R. Myler & I.J. Finkelstein (2017) Eukaryotic resectosomes: A single-molecule perspective. Prog Biophys Mol Bi